Anti-ATF5 Antibody
419.42 $ – 243.82 $
Immunohistochemical staining with rabbit anti-ATF5 antibody IGX-43520 at 1:100 in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Catalog No
IGX-43520
Supplier
ImuGeX
Size
100 μL < 50 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5′-GTGACGT[AC][AG]-3′), ATF5-specific response element (ARE) (consensus: 5′-C[CT]TCT[CT]CCTT[AT]-3′) but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PMID:10373550, PMID:15358120, PMID:21212266, PMID:20654631). Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4 (PMID:15358120). Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate . Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation . Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes . May be involved in osteogenic differentiation (PMID:22442021). Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1 (PMID:21791614). Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent . Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver (PMID:18332083). In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase (PMID:22528486, PMID:18701499). May act as a negative regulator of IL1B transduction pathway in liver (PMID:24379400). Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members (PMID:25512613). Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation (PMID:24216764). Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material (PMID:26213385). |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, IHC |
| Recommended Dilutions | IHC: 1:20-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Cyclic AMP-dependent transcription factor ATF-5 protein (1-282AA) |
| Specificity | ATF5 antibody detects endogenous level of ATF5 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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