Anti-botF Antibody
419.18 $ – 243.68 $
Western Blot
Positive WB detected in Recombinant protein
All lanes: botF antibody at 3.5µg/mL
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-49578
Supplier
ImuGeX
Size
100 μL < 50 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Botulinum neurotoxin type F: Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin F which may have 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol. Once in the cytosol the disulfide bond linking the 2 subunits is reduced and LC cleaves its target protein on synaptic vesicles, preventing their fusion with the cytoplasmic membrane and thus neurotransmitter release . Whole toxin only has protease activity after reduction, which releases LC (PMID:8505288). Requires complex eukaryotic host polysialogangliosides for full neurotoxicity . It is not clear whether a synaptic vesicle protein acts as its receptor; there is evidence for and against SV2 fulfilling this function . Botulinum neurotoxin F light chain: Has proteolytic activity (PMID:8505288, PMID:8175689, PMID:8197120). After translocation into the eukaryotic host cytosol, inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the ’60-Gln-|-Lys-61′ bond of synaptobrevin-1/VAMP1 and the equivalent ‘Gln-|-Lys’ sites in VAMP2 and VAMP3 (PMID:8505288, PMID:8175689). Cleaves the ’48-Gln-|-Lys-49′ bond of A.californica synaptobrevin (AC P35589) (PMID:8197120). Botulinum neurotoxin F heavy chain: Responsible for host epithelial cell transcytosis, host nerve cell targeting and translocation of light chain (LC) into host cytosol. Composed of 3 subdomains; the translocation domain (TD), and N-terminus and C-terminus of the receptor-binding domain (RBD). The RBD is responsible for the adherence of the toxin to the cell surface. It simultaneously recognizes 2 coreceptors; polysialated gangliosides and the receptor protein SV2A, SV2B and SV2C in close proximity on host synaptic vesicles; although not all evidence indicates these are the receptors . The N-terminus of the TD wraps an extended belt around the perimeter of the LC, protecting Zn2+ in the active site; it may also prevent premature LC dissociation from the translocation channel and protect toxin prior to translocation . The TD inserts into synaptic vesicle membrane to allow translocation into the host cytosol . |
| Host Species | Rabbit |
| Species Reactivity | Clostridium botulinum |
| Applications | ELISA, WB |
| Recommended Dilutions | WB: 1:500-1:5000 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Clostridium botulinum Botulinum neurotoxin type F protein (1-436AA) |
| Specificity | botF antibody detects endogenous level of botF protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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