Anti-CASP4 / Caspase 4 Antibody
417.46 $ – 242.68 $
Western Blot
analysis of Mouse kidney tissue, Mouse brain tissue, Mouse skeletal muscle tissue
All lanes: CASP4 antibody at 3.5µg/mL
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-51194
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Inflammatory caspase (PMID:7797510, PMID:23516580, PMID:25119034). Essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS (PMID:22246630, PMID:26174085, PMID:26173988, PMID:26508369, PMID:25964352). Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PMID:24879791, PMID:25964352). Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection (PMID:25121752). In later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity (PMID:26508369). Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer’s patient brains (PMID:15123740, PMID:22246630, PMID:23661706). Activated by direct binding to LPS without the need of an upstream sensor (PMID:25119034). Does not directly process IL1B (PMID:7743998, PMID:7797592, PMID:7797510). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PMID:28314590). |
| Host Species | Rabbit |
| Species Reactivity | Human, Mouse |
| Applications | ELISA, WB, IHC, IF |
| Recommended Dilutions | WB: 1:500-1:5000, IHC: 1:500-1:1000, IF: 1:50-1:500 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Caspase-4 protein (82-226AA) |
| Specificity | CASP4 antibody detects endogenous level of CASP4 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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