Anti-CDK2 / p33 protein kinase Antibody
419.70 $ – 243.98 $
IHC staining of paraffin-embedded Human cervical cancer tissue
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-52393
Supplier
ImuGeX
Size
100 μL < 50 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 . Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability . Phosphorylates CDK2AP2 (PMID:12944431). Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks (PMID:29203878). |
| Host Species | Rabbit |
| Species Reactivity | Human, Mouse, Rat |
| Applications | ELISA, WB, IHC |
| Recommended Dilutions | ELISA: 1:1000-1:2000, WB: 1:200-1:1000, IHC: 1:15-1:50 |
| Conjugate | Unconjugated |
| Immunogen | Fusion protein of human CDK2 |
| Specificity | CDK2 antibody detects endogenous level of CDK2 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.05% NaN3, 40% Glycerol |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.05% NaN3, 40% Glycerol |
| Formulation | Liquid |
| Purification | Affinity Purified |
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
Related Products
-
419.70 $ – 243.98 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.70 $ – 243.98 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.70 $ – 243.98 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.70 $ – 243.98 $Buy now This product has multiple variants. The options may be chosen on the product page