Anti-CUL4A / Cullin-4A Antibody
419.70 $ – 243.98 $
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Human fetal muscle tissue, Human fetal brain tissue, HeLa cells, Primary antibody: CUL4A antibody at dilution 1/325 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
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Catalog No
IGX-52830
Supplier
ImuGeX
Size
100 μL < 50 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL. The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PMID:26431207). With CUL4B, contributes to ribosome biogenesis (PMID:26711351). |
| Host Species | Rabbit |
| Species Reactivity | Human, Mouse |
| Applications | ELISA, WB |
| Recommended Dilutions | ELISA: 1:2000-1:5000, WB: 1:500-1:2000 |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide of human CUL4A |
| Specificity | CUL4A antibody detects endogenous level of CUL4A protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.05% NaN3, 40% Glycerol |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.05% NaN3, 40% Glycerol |
| Formulation | Liquid |
| Purification | Affinity Purified |
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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