Anti-DDB2 Antibody
418.43 $ – 243.25 $
Western Blot
analysis of Mouse liver tissue, Rat liver tissue
All lanes: DDB2 antibody at 4.9µg/mL
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-49442
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively (PMID:10882109, PMID:11278856, PMID:11705987, PMID:9892649, PMID:12732143, PMID:15882621, PMID:16473935, PMID:18593899). Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair (PMID:10882109, PMID:11278856, PMID:11705987, PMID:16260596, PMID:12944386, PMID:14751237). The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches (PMID:10882109, PMID:11278856, PMID:11705987, PMID:16260596, PMID:12944386). Also functions as the substrate recognition module for the DCX (DDB2-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1) (PMID:12732143, PMID:15882621, PMID:16473935, PMID:18593899, PMID:26572825). The DDB2-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage (PMID:16678110, PMID:16473935). The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair (PMID:16678110, PMID:16473935). The DDB2-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER (PMID:15882621). The DDB2-CUL4-ROC1 complex also ubiquitinates KAT7/HBO1 in response to DNA damage, leading to its degradation: recognizes KAT7/HBO1 following phosphorylation by ATR (PMID:26572825). Isoform D1: Inhibits UV-damaged DNA repair. Isoform D2: Inhibits UV-damaged DNA repair. |
| Host Species | Rabbit |
| Species Reactivity | Human, Rat, Mouse |
| Applications | ELISA, WB |
| Recommended Dilutions | WB: 1:500-1:5000 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human DNA damage-binding protein 2 protein (1-185AA) |
| Specificity | DDB2 antibody detects endogenous level of DDB2 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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