Anti-DDX11 Antibody
389.62 $ – 242.68 $
Immunohistochemistry of paraffin-embedded human tonsil tissue with rabbit anti-DDX11 antibody IGX-42865
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-42865
Supplier
ImuGeX
Size
100 μL < 50 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | DNA-dependent ATPase and ATP-dependent DNA helicase that participates in various functions in genomic stability, including DNA replication, DNA repair and heterochromatin organization as well as in ribosomal RNA synthesis (PMID:10648783, PMID:21854770, PMID:23797032, PMID:26089203, PMID:26503245). Its double-stranded DNA helicase activity requires either a minimal 5′-single-stranded tail length of approximately 15 nt (flap substrates) or 10 nt length single-stranded gapped DNA substrates of a partial duplex DNA structure for helicase loading and translocation along DNA in a 5′ to 3′ direction (PMID:18499658, PMID:22102414). The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended up to 500 bp by the replication protein A (RPA) or the cohesion CTF18-replication factor C (Ctf18-RFC) complex activities (PMID:18499658). Shows also ATPase- and helicase activities on substrates that mimic key DNA intermediates of replication, repair and homologous recombination reactions, including forked duplex, anti-parallel G-quadruplex and three-stranded D-loop DNA molecules (PMID:22102414, PMID:26503245). Plays a role in DNA double-strand break (DSB) repair at the DNA replication fork during DNA replication recovery from DNA damage (PMID:23797032). Recruited with TIMELESS factor upon DNA-replication stress response at DNA replication fork to preserve replication fork progression, and hence ensure DNA replication fidelity (PMID:26503245). Cooperates also with TIMELESS factor during DNA replication to regulate proper sister chromatid cohesion and mitotic chromosome segregation (PMID:17105772, PMID:18499658, PMID:20124417, PMID:23116066, PMID:23797032). Stimulates 5′-single-stranded DNA flap endonuclease activity of FEN1 in an ATP- and helicase-independent manner; and hence it may contribute in Okazaki fragment processing at DNA replication fork during lagging strand DNA synthesis (PMID:18499658). Its ability to function at DNA replication fork is modulated by its binding to long non-coding RNA (lncRNA) cohesion regulator non-coding RNA DDX11-AS1/CONCR, which is able to increase both DDX11 ATPase activity and binding to DNA replicating regions (PMID:27477908). Plays also a role in heterochromatin organization (PMID:21854770). Involved in rRNA transcription activation through binding to active hypomethylated rDNA gene loci by recruiting UBTF and the RNA polymerase Pol I transcriptional machinery (PMID:26089203). Plays a role in embryonic development and prevention of aneuploidy . Involved in melanoma cell proliferation and survival (PMID:23116066). Associates with chromatin at DNA replication fork regions (PMID:27477908). Binds to single- and double-stranded DNAs (PMID:9013641, PMID:18499658, PMID:22102414). |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, IHC |
| Recommended Dilutions | IHC: 1:20-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human ATP-dependent DNA helicase DDX11 protein (1-220AA) |
| Specificity | DDX11 antibody detects endogenous level of DDX11 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
Related Products
-
417.46 $ – 242.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
417.46 $ – 242.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
417.46 $ – 242.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
417.46 $ – 242.68 $Buy now This product has multiple variants. The options may be chosen on the product page