Anti-DNMT3L Antibody
419.42 $ – 243.82 $
Immunofluorescence staining of HeLa cells with rabbit anti-DNMT3L antibody IGX-49669
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-49669
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context . Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain (PMID:17687327). Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases (PMID:17687327). Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3 (PMID:17687327). Plays a key role in embryonic stem cells and germ cells . In germ cells, required for the methylation of imprinted loci together with DNMT3A . In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization . Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation . While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes . Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes . Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression . |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, WB, IF |
| Recommended Dilutions | WB: 1:500-1:5000, IF: 1:50-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human DNA (cytosine-5)-methyltransferase 3-like protein (1-234AA) |
| Specificity | DNMT3L antibody detects endogenous level of DNMT3L protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
Related Products
-
419.42 $ – 243.82 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.42 $ – 243.82 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.42 $ – 243.82 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.42 $ – 243.82 $Buy now This product has multiple variants. The options may be chosen on the product page