Anti-EGLN2 Antibody
419.70 $ – 243.98 $
Immunohistochemistry of paraffin-embedded human lung tissue with rabbit anti-EGLN2 antibody IGX-43653 at dilution 1:100.
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Catalog No
IGX-43653
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as ATF4, IKBKB, CEP192 and HIF1A (PMID:11595184, PMID:12039559, PMID:15925519, PMID:16509823, PMID:17114296, PMID:23932902). Target proteins are preferentially recognized via a LXXLAP motif (PMID:11595184, PMID:12039559, PMID:15925519). Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins (PMID:11595184, PMID:12039559, PMID:12181324, PMID:15925519, PMID:19339211). Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A (PMID:11595184, PMID:12039559, PMID:12181324, PMID:15925519). Also hydroxylates HIF2A (PMID:11595184, PMID:12039559, PMID:15925519). Has a preference for the CODD site for both HIF1A and HIF2A (PMID:11595184, PMID:12039559, PMID:15925519). Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex (PMID:11595184, PMID:12039559, PMID:15925519). Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes (PMID:11595184, PMID:12039559, PMID:15925519). EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle (PMID:11595184, PMID:12039559, PMID:15925519). Also regulates susceptibility to normoxic oxidative neuronal death (PMID:11595184, PMID:12039559, PMID:15925519). Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation (PMID:23932902). Hydroxylates IKBKB, mediating NF-kappa-B activation in hypoxic conditions (PMID:17114296). Also mediates hydroxylation of ATF4, leading to decreased protein stability of ATF4 . |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, IHC, IF |
| Recommended Dilutions | IHC: 1:20-1:200, IF: 1:50-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Egl nine homolog 2 protein (283-407AA) |
| Specificity | EGLN2 antibody detects endogenous level of EGLN2 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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