Anti-Egln3 Antibody
419.18 $ – 243.68 $
Western Blot
analysis of Rat brain tissue, Mouse brain tissue
All lanes: Egln3 antibody at 3.6µg/mL
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-49190
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as PKM, TELO2, ATF4 and HIF1A (PMID:24809345). Target proteins are preferentially recognized via a LXXLAP motif . Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins . Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A . Also hydroxylates HIF2A . Has a preference for the CODD site for both HIF1A and HIF2A . Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site . Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex . Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes . ELGN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia . Also hydroxylates PKM in hypoxia, limiting glycolysis . Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis . In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex . In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity . Also essential for hypoxic regulation of neutrophilic inflammation (PMID:21317538). Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway . Also mediates hydroxylation of ATF4, leading to decreased protein stability of ATF4 (PMID:24809345). |
| Host Species | Rabbit |
| Species Reactivity | Mouse, Rat |
| Applications | ELISA, WB |
| Recommended Dilutions | WB: 1:500-1:5000 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Mouse Egl nine homolog 3 protein (2-239AA) |
| Specificity | Egln3 antibody detects endogenous level of Egln3 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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