Anti-EIF2AK1 Antibody
419.18 $ – 243.68 $
Western Blot
analysis of HepG2 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Rat heart tissue, Rat lung tissue
All lanes: EIF2AK1 antibody at 3µg/mL
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-50568
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions (PMID:32132706, PMID:32132707). Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as heme deficiency, oxidative stress, osmotic shock, mitochondrial dysfunction and heat shock (PMID:32132706, PMID:32132707). EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming (PMID:32132706, PMID:32132707). Acts as a key sensor of heme-deficiency: in normal conditions, binds hemin via a cysteine thiolate and histidine nitrogenous coordination, leading to inhibit the protein kinase activity . This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell: heme depletion relieves inhibition and stimulates kinase activity, activating the ISR . Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions . In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties . It thereby plays an essential protective role for RBC survival in anemias of iron deficiency . Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes . May also regulate endoplasmic reticulum (ER) stress during acute heme-deficient conditions . Also activates the ISR in response to mitochondrial dysfunction: HRI/EIF2AK1 protein kinase activity is activated upon binding to the processed form of DELE1 (S-DELE1), thereby promoting the ATF4-mediated reprogramming (PMID:32132706, PMID:32132707). |
| Host Species | Rabbit |
| Species Reactivity | Human, Rat |
| Applications | ELISA, WB, IHC, IF |
| Recommended Dilutions | WB: 1:500-1:5000, IHC: 1:20-1:200, IF: 1:50-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Eukaryotic translation initiation factor 2-alpha kinase 1 protein (1-86AA) |
| Specificity | EIF2AK1 antibody detects endogenous level of EIF2AK1 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
Related Products
-
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page