Anti-EIF2S1 (Ab-51) / eIF2a Antibody
419.42 $
Western blot analysis of extracts from 3T3, HeLa and C6 cells EIF2S1 (Ab-51) / eIF2a antibody
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Catalog No
IGX-53175
Supplier
ImuGeX
Size
100 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PMID:16289705). This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (PMID:16289705). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex (PMID:16289705). In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (PMID:16289705). EIF2S1/eIF-2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming (PMID:19131336). |
| Host Species | Rabbit |
| Species Reactivity | Human, Mouse, Rat |
| Applications | ELISA, WB, IHC, IF |
| Recommended Dilutions | WB: 1:500-1:1000, IHC: 1:50-1:200, IF: 1:100-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Peptide sequence around AA 49~53 (E-L-S-R-R) derived from Human eIF2a. |
| Specificity | EIF2S1 (Ab-51) antibody detects endogenous level of EIF2S1 (Ab-51) protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Formulation | Liquid |
| Purification | Affinity Purified |
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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