Anti-EIF4A3 Antibody
419.28 $ – 243.74 $
Western Blot
analysis of A549 whole cell lysate
All lanes: EIF4A3 antibody at 3µg/mL
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-47501
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | ATP-dependent RNA helicase (PMID:16170325). Involved in pre-mRNA splicing as component of the spliceosome (PMID:11991638, PMID:22961380, PMID:28502770, PMID:28076346, PMID:29301961). Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PMID:16209946, PMID:16170325, PMID:16314458, PMID:16923391, PMID:16931718, PMID:19033377, PMID:20479275). The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly (PMID:22203037). Involved in craniofacial development (PMID:24360810). |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, WB, IHC, IF |
| Recommended Dilutions | WB: 1:1000-1:5000, IHC: 1:20-1:200, IF: 1:50-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Eukaryotic initiation factor 4A-III protein (6-166AA) |
| Specificity | EIF4A3 antibody detects endogenous level of EIF4A3 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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