Anti-ITCH Antibody
419.42 $ – 243.82 $
Western Blot
analysis of K562 whole cell lysate, 293 whole cell lysate, HeLa whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate
All lanes: ITCH antibody at 1:2000
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-46436
Supplier
ImuGeX
Size
100 μL < 50 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PMID:14602072, PMID:17028573, PMID:16387660, PMID:18718448, PMID:18718449, PMID:11046148, PMID:19592251, PMID:19116316, PMID:19881509, PMID:20491914, PMID:20392206, PMID:20068034, PMID:23146885, PMID:24790097, PMID:25631046). Catalyzes ‘Lys-29’-, ‘Lys-48’- and ‘Lys-63’-linked ubiquitin conjugation (PMID:17028573, PMID:18718448, PMID:19131965, PMID:19881509). Involved in the control of inflammatory signaling pathways (PMID:19131965). Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways (PMID:19131965). Promotes the association of the complex after TNF stimulation (PMID:19131965). Once the complex is formed, TNFAIP3 deubiquitinates ‘Lys-63’ polyubiquitin chains on RIPK1 and catalyzes the formation of ‘Lys-48’-polyubiquitin chains (PMID:19131965). This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1 (PMID:19131965). Ubiquitinates RIPK2 by ‘Lys-63’-linked conjugation and influences NOD2-dependent signal transduction pathways (PMID:19592251). Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response (PMID:18718448, PMID:20491914). Ubiquitinates NFE2 by ‘Lys-63’ linkages and is implicated in the control of the development of hematopoietic lineages (PMID:18718448). Mediates JUN ubiquitination and degradation . Mediates JUNB ubiquitination and degradation (PMID:16387660). Critical regulator of type 2 helper T (Th2) cell cytokine production by inducing JUNB ubiquitination and degradation . Involved in the negative regulation of MAVS-dependent cellular antiviral responses (PMID:19881509). Ubiquitinates MAVS through ‘Lys-48’-linked conjugation resulting in MAVS proteasomal degradation (PMID:19881509). Following ligand stimulation, regulates sorting of Wnt receptor FZD4 to the degradative endocytic pathway probably by modulating PI42KA activity (PMID:23146885). Ubiquitinates PI4K2A and negatively regulates its catalytic activity (PMID:23146885). Ubiquitinates chemokine receptor CXCR4 and regulates sorting of CXCR4 to the degradative endocytic pathway following ligand stimulation by ubiquitinating endosomal sorting complex required for transport ESCRT-0 components HGS and STAM (PMID:14602072, PMID:23146885). Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through ‘Lys-29’-linked polyubiquitination (PMID:17028573, PMID:18628966, PMID:23886940). Ubiquitinates SNX9 (PMID:20491914). Ubiquitinates MAP3K7 through ‘Lys-48’-linked conjugation . Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP (PMID:20068034). Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteasomal degradation of p15 BID (PMID:20392206). Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PMID:25631046). |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, WB, IF |
| Recommended Dilutions | WB: 1:1000-1:5000, IF: 1:50-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human E3 ubiquitin-protein ligase Itchy homolog protein (29-303AA) |
| Specificity | ITCH antibody detects endogenous level of ITCH protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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