Anti-JAK2 (Ab-570) Antibody
421.25 $
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Jak2 (Ab-570) antibody (left) and the same antibody preincubated with blocking peptide (right).
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Catalog No
IGX-54290
Supplier
ImuGeX
Size
100 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PMID:7615558). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PMID:9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B) (PMID:21368206). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PMID:20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PMID:21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of ‘Tyr-41’ of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PMID:19783980). |
| Host Species | Rabbit |
| Species Reactivity | Human, Rat |
| Applications | ELISA, IHC |
| Recommended Dilutions | IHC: 1:50-1:100 |
| Conjugate | Unconjugated |
| Immunogen | Peptide sequence around AA 568~572 (G-D-Y-G-Q) derived from Human JAK2. |
| Specificity | JAK2 (Ab-570) antibody detects endogenous level of JAK2 (Ab-570) protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Formulation | Liquid |
| Purification | Affinity Purified |
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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