Anti-JMJD6 Antibody
421.25 $ – 244.89 $
IHC staining of paraffin-embedded Human brain tissue
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Catalog No
IGX-54295
Supplier
ImuGeX
Size
100 μL < 50 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Dioxygenase that can both act as a arginine demethylase and a lysyl-hydroxylase (PMID:24498420, PMID:17947579, PMID:20684070, PMID:21060799, PMID:22189873). Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Regulates RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65 (PMID:19574390). Hydroxylates its own N-terminus, which is required for homooligomerization (PMID:22189873). In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA (PMID:20679243, PMID:29176719). Also acts as an arginine demethylase which preferentially demethylates asymmetric dimethylation (PMID:17947579, PMID:24498420, PMID:24360279). Demethylates histone H3 at ‘Arg-2’ (H3R2me) and histone H4 at ‘Arg-3’ (H4R3me), including mono-, symmetric di- and asymmetric dimethylated forms, thereby playing a role in histone code (PMID:17947579, PMID:24360279). However, histone arginine demethylation may not constitute the primary activity in vivo (PMID:17947579, PMID:21060799, PMID:22189873). In collaboration with BRD4, interacts with the positive transcription elongation factor b (P-TEFb) complex in its active form to regulate polymerase II promoter-proximal pause release for transcriptional activation of a large cohort of genes. On distal enhancers, so called anti-pause enhancers, demethylates both histone H4R3me2 and the methyl cap of 7SKsnRNA leading to the dismissal of the 7SKsnRNA:HEXIM1 inhibitor complex. After removal of repressive marks, the complex BRD4:JMJD6 attract and retain the P-TEFb complex on chromatin, leading to its activation, promoter-proximal polymerase II pause release, and transcriptional activation (PMID:24360279). Demethylates other arginine methylated-proteins such as ESR1 (PMID:24498420). Has no histone lysine demethylase activity (PMID:21060799). Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65 . Seems to be necessary for the regulation of macrophage cytokine responses (PMID:15622002). |
| Host Species | Rabbit |
| Species Reactivity | Human, Mouse, Rat |
| Applications | ELISA, WB, IHC |
| Recommended Dilutions | ELISA: 1:2000-1:5000, WB: 1:500-1:2000, IHC: 1:50-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Fusion protein of human JMJD6 |
| Specificity | JMJD6 antibody detects endogenous level of JMJD6 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.05% NaN3, 40% Glycerol |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.05% NaN3, 40% Glycerol |
| Formulation | Liquid |
| Purification | Affinity Purified |
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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