Anti-KAT7 Antibody
419.18 $ – 243.68 $
Western blot
All lanes: KAT7 antibody at 2µg/mL + Rat brain tissue
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Catalog No
IGX-49928
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Catalytic subunit of histone acetyltransferase HBO1 complexes, which specifically mediate acetylation of histone H3 at ‘Lys-14’ (H3K14ac), thereby regulating various processes, such as gene transcription, protein ubiquitination, immune regulation, stem cell pluripotent and self-renewal maintenance and embryonic development (PMID:16387653, PMID:21753189, PMID:24065767, PMID:26620551, PMID:31767635, PMID:31827282). Some complexes also catalyze acetylation of histone H4 at ‘Lys-5’, ‘Lys-8’ and ‘Lys-12’ (H4K5ac, H4K8ac and H4K12ac, respectively), regulating DNA replication initiation, regulating DNA replication initiation (PMID:10438470, PMID:19187766, PMID:20129055, PMID:24065767). Specificity of the HBO1 complexes is determined by the scaffold subunit: complexes containing BRPF scaffold (BRPF1, BRD1/BRPF2 or BRPF3) direct KAT7/HBO1 specificity towards H3K14ac, while complexes containing JADE (JADE1, JADE2 and JADE3) scaffold direct KAT7/HBO1 specificity towards histone H4 (PMID:19187766, PMID:20129055, PMID:24065767, PMID:26620551). H3K14ac promotes transcriptional elongation by facilitating the processivity of RNA polymerase II (PMID:31827282). Acts as a key regulator of hematopoiesis by forming a complex with BRD1/BRPF2, directing KAT7/HBO1 specificity towards H3K14ac and promoting erythroid differentiation (PMID:21753189). H3K14ac is also required for T-cell development . KAT7/HBO1-mediated acetylation facilitates two consecutive steps, licensing and activation, in DNA replication initiation: H3K14ac facilitates the activation of replication origins, and histone H4 acetylation (H4K5ac, H4K8ac and H4K12ac) facilitates chromatin loading of MCM complexes, promoting DNA replication licensing (PMID:10438470, PMID:11278932, PMID:18832067, PMID:19187766, PMID:20129055, PMID:21856198, PMID:24065767, PMID:26620551). Acts as a positive regulator of centromeric CENPA assembly: recruited to centromeres and mediates histone acetylation, thereby preventing centromere inactivation mediated by SUV39H1, possibly by increasing histone turnover/exchange (PMID:27270040). Involved in nucleotide excision repair: phosphorylation by ATR in response to ultraviolet irradiation promotes its localization to DNA damage sites, where it mediates histone acetylation to facilitate recruitment of XPC at the damaged DNA sites (PMID:28719581). Acts as an inhibitor of NF-kappa-B independently of its histone acetyltransferase activity (PMID:16997280). Plays a central role in the maintenance of leukemia stem cells in acute myeloid leukemia (AML) (PMID:31827282). Acts by mediating acetylation of histone H3 at ‘Lys-14’ (H3K14ac), thereby facilitating the processivity of RNA polymerase II to maintain the high expression of key genes, such as HOXA9 and HOXA10 that help to sustain the functional properties of leukemia stem cells (PMID:31827282). |
| Host Species | Rabbit |
| Species Reactivity | Human, Rat |
| Applications | ELISA, WB, IHC |
| Recommended Dilutions | WB: 1:500-1:5000, IHC: 1:20-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Histone acetyltransferase KAT7 protein (1-214AA) |
| Specificity | KAT7 antibody detects endogenous level of KAT7 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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