Anti-LARP7 Antibody
419.42 $ – 243.82 $
Immunohinstochemical staining with rabbit anti-LARP7 antibody IGX-44993 at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-44993
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | RNA-binding protein that specifically binds distinct small nuclear RNA (snRNAs) and regulates their processing and function (PMID:18249148, PMID:32017898). Specifically binds the 7SK snRNA (7SK RNA) and acts as a core component of the 7SK ribonucleoprotein (RNP) complex, thereby acting as a negative regulator of transcription elongation by RNA polymerase II (PMID:18249148, PMID:18483487). The 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation (PMID:18249148, PMID:18483487). The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC) (PMID:28254838). LARP7 specifically binds to the highly conserved 3′-terminal U-rich stretch of 7SK RNA; on stimulation, remains associated with 7SK RNA, whereas P-TEFb is released from the complex (PMID:18483487, PMID:18281698). LARP7 also acts as a regulator of mRNA splicing fidelity by promoting U6 snRNA processing (PMID:32017898). Specifically binds U6 snRNAs and associates with a subset of box C/D RNP complexes: promotes U6 snRNA 2′-O-methylation by facilitating U6 snRNA loading into box C/D RNP complexes (PMID:32017898). U6 snRNA 2′-O-methylation is required for mRNA splicing fidelity (PMID:32017898). Binds U6 snRNAs with a 5′-CAGGG-3′ sequence motif (PMID:32017898). U6 snRNA processing is required for spermatogenesis . |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, IHC |
| Recommended Dilutions | IHC: 1:200-1:500 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human La-related protein 7 protein (409-471AA) |
| Specificity | LARP7 antibody detects endogenous level of LARP7 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
Related Products
-
419.42 $ – 243.82 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.42 $ – 243.82 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.42 $ – 243.82 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.42 $ – 243.82 $Buy now This product has multiple variants. The options may be chosen on the product page