Anti-LIN28B Antibody
419.28 $
Western blot analysis of extracts from HeLa and 293 cells
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-54573
Supplier
ImuGeX
Size
100 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PMID:22118463). Does not act on pri-miR21 (PMID:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PMID:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PMID:22118463). Interaction with target pre-miRNAs occurs via an 5′-GGAG-3′ motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression . When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, WB |
| Recommended Dilutions | WB: 1:500-1:1000 |
| Conjugate | Unconjugated |
| Immunogen | Peptide sequence around AA 242~246 ( P-S-V-Q-K) derived from Human LIN28B |
| Specificity | LIN28B antibody detects endogenous level of LIN28B protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Formulation | Liquid |
| Purification | Affinity Purified |
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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