Anti-LOXL2 Antibody
419.70 $ – 243.98 $
Western Blot
analysis of Rat lung tissue, Rat liver tissue, Mouse heart tissue, Mouse lung tissue
All lanes: LOXL2 antibody at 3.5µg/mL
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-50173
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine) (PMID:27735137). Acts as a transcription corepressor and specifically mediates deamination of trimethylated ‘Lys-4’ of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation (PMID:27735137). Shows no activity against histone H3 when it is trimethylated on ‘Lys-9’ (H3K9me3) or ‘Lys-27’ (H3K27me3) or when ‘Lys-4’ is monomethylated (H3K4me1) or dimethylated (H3K4me2) (PMID:27735137). Also mediates deamination of methylated TAF10, a member of the transcription factor IID (TFIID) complex, which induces release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription (PMID:25959397). LOXL2-mediated deamination of TAF10 results in transcriptional repression of genes required for embryonic stem cell pluripotency including POU5F1/OCT4, NANOG, KLF4 and SOX2 . Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E-cadherin CDH1, probably by mediating deamination of histone H3 (PMID:16096638, PMID:27735137, PMID:24414204). During EMT, involved with SNAI1 in negatively regulating pericentromeric heterochromatin transcription (PMID:24239292). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (PMID:24239292). Interacts with the endoplasmic reticulum protein HSPA5 which activates the IRE1-XBP1 pathway of the unfolded protein response, leading to expression of several transcription factors involved in EMT and subsequent EMT induction (PMID:28332555). Involved in E-cadherin repression following hypoxia, a hallmark of EMT believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression (PMID:20026874). When secreted into the extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin (PMID:20306300). Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding (PMID:21835952). Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation . |
| Host Species | Rabbit |
| Species Reactivity | Human, Mouse, Rat |
| Applications | ELISA, WB, IHC |
| Recommended Dilutions | WB: 1:500-1:5000, IHC: 1:20-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Lysyl oxidase homolog 2 protein (27-121AA) |
| Specificity | LOXL2 antibody detects endogenous level of LOXL2 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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