Anti-MAF1 Antibody
419.42 $ – 243.82 $
Western blot
All lanes: MAF1 antibody at 2µg/mL,
Lane 1: MCF-7 whole cell lysate
Lane 2: PC-3 whole cell lysate
Lane 3: Mouse kidney tissue
Lane 4: A431 whole cell lysate
Secondary:
Goat polyclonal to rabbit IgG at 1/10000 dilution
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-46182
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Plays a role in the repression of RNA polymerase III-mediated transcription in response to changing nutritional, environmental and cellular stress conditions to balance the production of highly abundant tRNAs, 5S rRNA, and other small non-coding RNAs with cell growth and maintenance (PMID:18377933, PMID:20233713, PMID:20516213, PMID:20543138). Plays also a key role in cell fate determination by promoting mesorderm induction and adipocyte differentiation . Mechanistically, associates with the RNA polymerase III clamp and thereby impairs its recruitment to the complex made of the promoter DNA, TBP and the initiation factor TFIIIB (PMID:20887893, PMID:17505538). When nutrients are available and mTOR kinase is active, MAF1 is hyperphosphorylated and RNA polymerase III is engaged in transcription. Stress-induced MAF1 dephosphorylation results in nuclear localization, increased targeting of gene-bound RNA polymerase III and a decrease in the transcriptional readout (PMID:26941251). Additionally, may also regulate RNA polymerase I and RNA polymerase II-dependent transcription through its ability to regulate expression of the central initiation factor TBP (PMID:17499043). |
| Host Species | Rabbit |
| Species Reactivity | Human, Mouse |
| Applications | ELISA, WB |
| Recommended Dilutions | WB: 1:1000-1:5000 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Repressor of RNA polymerase III transcription MAF1 homolog protein (1-256AA) |
| Specificity | MAF1 antibody detects endogenous level of MAF1 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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