Anti-MAGOH Antibody
391.72 $ – 243.98 $
Western blot
All lanes: MAGOH antibody at 4.36µg/mL
Lane 1: Mouse kidney tissue
Lane 2: A431 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: Raji whole cell lysate
Lane 5: K562 whole cell lysate
Lane 6: HeLa whole cell lysate
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-47702
Supplier
ImuGeX
Size
100 μL < 50 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Required for pre-mRNA splicing as component of the spliceosome (PMID:11991638). Plays a redundant role with MAGOHB as core component of the exon junction complex (EJC) and in the nonsense-mediated decay (NMD) pathway (PMID:23917022). The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. |
| Host Species | Rabbit |
| Species Reactivity | Human, Mouse |
| Applications | ELISA, WB, IP |
| Recommended Dilutions | WB: 1:1000-1:5000, IP: 1:200-1:2000 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Protein mago nashi homolog protein (1-146AA) |
| Specificity | MAGOH antibody detects endogenous level of MAGOH protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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