Anti-MAPK8 Antibody
419.28 $ – 243.74 $
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: K562 and HeLa cell, Primary antibody: MAPK8 antibody at dilution 1/600 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
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Catalog No
IGX-54757
Supplier
ImuGeX
Size
100 μL < 50 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PMID:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PMID:10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1’s transcriptional activity and enhances its proteosomal degradation . Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 . In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses . JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. |
| Host Species | Rabbit |
| Species Reactivity | Human, Mouse, Rat |
| Applications | ELISA, WB |
| Recommended Dilutions | ELISA: 1:2000-1:10000, WB: 1:1000-1:5000 |
| Conjugate | Unconjugated |
| Immunogen | Fusion protein of human MAPK8 |
| Specificity | MAPK8 antibody detects endogenous level of MAPK8 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.05% NaN3, 40% Glycerol |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.05% NaN3, 40% Glycerol |
| Formulation | Liquid |
| Purification | Affinity Purified |
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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