Anti-MAPK9 Antibody
419.18 $ – 243.68 $
Immunofluorescence staining of HeLa cells with rabbit anti-MAPK9 antibody IGX-40226
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-40226
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PMID:22441692). Phosphorylates POU5F1, which results in the inhibition of POU5F1’s transcriptional activity and enhances its proteosomal degradation . MAPK9 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, IF, IP |
| Recommended Dilutions | IF: 1:200-1:500, IP: 1:200-1:2000 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Mitogen-activated protein kinase 9 protein (1-424AA) |
| Specificity | MAPK9 antibody detects endogenous level of MAPK9 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
Related Products
-
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page