Anti-NPHP4 Antibody
419.18 $ – 243.68 $
Immunohinstochemical staining with rabbit anti-NPHP4 antibody IGX-45614 at 1:500 in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-45614
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Involved in the organization of apical junctions; the function is proposed to implicate a NPHP1-4-8 module (PMID:19755384, PMID:21565611). Does not seem to be strictly required for ciliogenesis (PMID:21565611). Required for building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells. Seems to recruit INT to basal bodies of motile cilia which subsequently interacts with actin-modifying proteins such as DAAM1 . In cooperation with INVS may downregulate the canonical Wnt pathway and promote the Wnt-PCP pathway by regulating expression and subcellular location of disheveled proteins. Stabilizes protein levels of JADE1 and promotes its translocation to the nucleus leading to cooperative inhibition of canonical Wnt signaling (PMID:21498478, PMID:22654112). Acts as negative regulator of the hippo pathway by association with LATS1 and modifying LATS1-dependent phosphorylation and localization of WWTR1/TAZ (PMID:21555462). |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, IHC, IF |
| Recommended Dilutions | IHC: 1:500-1:1000, IF: 1:50-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Nephrocystin-4 protein (397-543AA) |
| Specificity | NPHP4 antibody detects endogenous level of NPHP4 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
Related Products
-
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page