Anti-NSUN2 Antibody
419.28 $ – 243.74 $
Western Blot
analysis of HepG2 whole cell lysate, Rat liver tissue, Mouse spleen tissue
All lanes: NSUN2 antibody at 6.7µg/mL
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-50958
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | RNA cytosine C5-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs) (PMID:17071714, PMID:22995836, PMID:31358969, PMID:31199786). Involved in various processes, such as epidermal stem cell differentiation, testis differentiation and maternal to zygotic transition during early development: acts by increasing protein synthesis; cytosine C5-methylation promoting tRNA stability and preventing mRNA decay (PMID:31199786). Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors (PMID:17071714, PMID:22995836, PMID:31199786). tRNA methylation is required generation of RNA fragments derived from tRNAs (tRFs) (PMID:31199786). Also mediates C5-methylation of mitochondrial tRNAs (PMID:31276587). Catalyzes cytosine C5-methylation of mRNAs, leading to stabilize them and prevent mRNA decay: mRNA stabilization involves YBX1 that specifically recognizes and binds m5C-modified transcripts (PMID:22395603, PMID:31358969). Cytosine C5-methylation of mRNAs also regulates mRNA export: methylated transcripts are specifically recognized by THOC4/ALYREF, which mediates mRNA nucleo-cytoplasmic shuttling (PMID:28418038). Also mediates cytosine C5-methylation of non-coding RNAs, such as vault RNAs (vtRNAs), promoting their processing into regulatory small RNAs (PMID:23871666). Cytosine C5-methylation of vtRNA VTRNA1.1 promotes its processing into small-vault RNA4 (svRNA4) and regulates epidermal differentiation (PMID:31186410). May act downstream of Myc to regulate epidermal cell growth and proliferation . Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity (PMID:19596847). |
| Host Species | Rabbit |
| Species Reactivity | Human, Rat, Mouse |
| Applications | ELISA, WB, IHC, IF |
| Recommended Dilutions | WB: 1:500-1:5000, IHC: 1:200-1:500, IF: 1:50-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human tRNA (cytosine(34)-C(5))-methyltransferase protein (432-509AA) |
| Specificity | NSUN2 antibody detects endogenous level of NSUN2 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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