Anti-NUMA1 Antibody
419.42 $ – 243.82 $
Western Blot
analysis of HeLa whole cell lysate, K562 whole cell lysate
All lanes: NUMA1 antibody at 3.2µg/mL
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-50665
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division (PMID:7769006, PMID:17172455, PMID:19255246, PMID:24996901, PMID:26195665, PMID:27462074). Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles (PMID:12445386, PMID:11956313). Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner (PMID:23870127, PMID:24109598, PMID:24996901, PMID:26765568). In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle (PMID:23027904, PMID:22327364, PMID:23921553). During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation (PMID:22327364, PMID:23921553, PMID:24996901, PMID:24371089). Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro (PMID:24996901, PMID:24371089). Also required for proper orientation of the mitotic spindle during asymmetric cell divisions (PMID:21816348). Plays a role in mitotic MT aster assembly (PMID:11163243, PMID:11229403, PMID:12445386). Involved in anastral spindle assembly (PMID:25657325). Positively regulates TNKS protein localization to spindle poles in mitosis (PMID:16076287). Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume (PMID:10075938). Required for epidermal differentiation and hair follicle morphogenesis . |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, WB, IHC, IF |
| Recommended Dilutions | WB: 1:500-1:5000, IHC: 1:20-1:200, IF: 1:50-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Nuclear mitotic apparatus protein 1 protein (627-870AA) |
| Specificity | NUMA1 antibody detects endogenous level of NUMA1 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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