Anti-PIWIL4 Antibody
419.18 $ – 243.68 $
Western Blot
analysis of HEK293 whole cell lysate
All lanes: PIWIL4 antibody at 3.4µg/mL
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-49709
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity . Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons . Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements . Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association . The piRNA process acts upstream of known mediators of DNA methylation . Does not show endonuclease activity . Plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a ‘slicer-incompetent’ component that loads cleaved piRNAs from the ‘slicer-competent’ component PIWIL2 and target them on genomic transposon loci in the nucleus . May be involved in the chromatin-modifying pathway by inducing ‘Lys-9’ methylation of histone H3 at some loci (PMID:17544373). In addition to its role in germline, PIWIL4 also plays a role in the regulation of somatic cells activities. Plays a role in pancreatic beta cell function and insulin secretion . Involved in maintaining cell morphology and functional integrity of retinal epithelial through Akt/GSK3alpha/beta signaling pathway (PMID:28025795). When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of cells proliferation in tumors (PMID:22483988). |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, WB, IF |
| Recommended Dilutions | WB: 1:500-1:5000, IF: 1:50-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Piwi-like protein 4 protein (12-235AA) |
| Specificity | PIWIL4 antibody detects endogenous level of PIWIL4 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
Related Products
-
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page -
419.18 $ – 243.68 $Buy now This product has multiple variants. The options may be chosen on the product page