Anti-PPIE Antibody
417.46 $ – 242.68 $
Immunohinstochemical staining with rabbit anti-PPIE antibody IGX-44974 at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Catalog No
IGX-44974
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Involved in pre-mRNA splicing as component of the spliceosome (PMID:11991638, PMID:28076346). Combines RNA-binding and PPIase activities (PMID:8977107, PMID:18258190, PMID:20677832, PMID:20460131). Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3′-UTR of mRNA molecules (PMID:8977107, PMID:18258190, PMID:20460131). Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins (PMID:8977107, PMID:18258190, PMID:20677832, PMID:20541251). Inhibits KMT2A activity; this requires proline isomerase activity (PMID:20677832, PMID:20541251, PMID:20460131). |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, IHC |
| Recommended Dilutions | IHC: 1:200-1:500 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Peptidyl-prolyl cis-trans isomerase E protein (86-141AA) |
| Specificity | PPIE antibody detects endogenous level of PPIE protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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