Anti-PRKACA Antibody
419.18 $ – 243.68 $
Western Blot
analysis of HeLa whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate
All lanes: PRKACA antibody at 1:2000
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-47628
Supplier
ImuGeX
Size
100 μL < 50 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Phosphorylates a large number of substrates in the cytoplasm and the nucleus (PMID:15642694, PMID:15905176, PMID:16387847, PMID:17333334, PMID:17565987, PMID:17693412, PMID:18836454, PMID:19949837, PMID:20356841, PMID:21085490, PMID:21514275, PMID:21812984). Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, SOX9 and VASP (PMID:15642694, PMID:15905176, PMID:16387847, PMID:17333334, PMID:17565987, PMID:17693412, PMID:18836454, PMID:19949837, PMID:20356841, PMID:21085490, PMID:21514275, PMID:21812984). Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (PMID:21423175). RORA is activated by phosphorylation (PMID:21514275). Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts (PMID:19949837). Involved in chondrogenesis by mediating phosphorylation of SOX9 . Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP (PMID:15642694, PMID:20356841). Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated (PMID:17333334). RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca2+, leading to reduced amplitude and increased frequency of store overload-induced Ca2+ release (SOICR) characterized by an increased rate of Ca2+ release and propagation velocity of spontaneous Ca2+ waves, despite reduced wave amplitude and resting cytosolic Ca2+ (PMID:17693412). PSMC5/RPT6 activation by phosphorylation stimulates proteasome (PMID:17565987). Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation (PMID:15905176). NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding (PMID:15642694). Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation . May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT) . Phosphorylates APOBEC3G and AICDA (PMID:16387847, PMID:18836454). Phosphorylates HSF1; this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock (PMID:21085490). Isoform 2: Phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, WB, IHC |
| Recommended Dilutions | WB: 1:1000-1:5000, IHC: 1:200-1:500 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human cAMP-dependent protein kinase catalytic subunit alpha protein (233-351AA) |
| Specificity | PRKACA antibody detects endogenous level of PRKACA protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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