Anti-PRKDC Antibody
418.43 $ – 243.25 $
Western Blot
Positive WB detected in Recombinant protein
All lanes: PRKDC antibody at 3µg/mL
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-49260
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination (PMID:11955432, PMID:12649176, PMID:14734805). Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C) (PMID:11955432). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step (PMID:15574326, PMID:11955432, PMID:12649176, PMID:14734805). Required to protect and align broken ends of DNA (PMID:15574326, PMID:11955432, PMID:12649176, PMID:14734805). May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage (PMID:15574326, PMID:11955432, PMID:12649176, PMID:14734805). Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription (PMID:15574326, PMID:11955432, PMID:12649176, PMID:14734805). As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome (PMID:32103174). Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome (PMID:32103174). Recognizes the substrate consensus sequence [ST]-Q (PMID:15574326, PMID:11955432, PMID:12649176, PMID:14734805). Phosphorylates ‘Ser-139’ of histone variant H2AX, thereby regulating DNA damage response mechanism (PMID:14627815, PMID:16046194). Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, FH, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2 (PMID:2507541, PMID:2247066, PMID:1597196, PMID:8407951, PMID:8464713, PMID:9362500, PMID:9139719, PMID:10026262, PMID:10467406, PMID:12509254, PMID:11889123, PMID:14612514, PMID:14704337, PMID:16397295, PMID:26237645, PMID:28712728). Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA (PMID:9679063). Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D (PMID:9363941). Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 ‘Ser-588’ and increasing CRY1 protein stability, most likely through an indirect mechanism . Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PMID:28712728). |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, WB |
| Recommended Dilutions | WB: 1:500-1:5000 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human DNA-dependent protein kinase catalytic subunit protein (3747-4015AA) |
| Specificity | PRKDC antibody detects endogenous level of PRKDC protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
Related Products
-
418.43 $ – 243.25 $Buy now This product has multiple variants. The options may be chosen on the product page -
418.43 $ – 243.25 $Buy now This product has multiple variants. The options may be chosen on the product page -
418.43 $ – 243.25 $Buy now This product has multiple variants. The options may be chosen on the product page -
418.43 $ – 243.25 $Buy now This product has multiple variants. The options may be chosen on the product page