Anti-PRKN Antibody
419.18 $ – 243.68 $
Western Blot
analysis of HepG2 whole cell lysate, SH-SY5Y whole cell lysate, Jurkat whole cell lysate
All lanes: PRKN antibody at 3µg/mL
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-51189
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPTIN5, TOMM20, USP30, ZNF746 and AIMP2 (PMID:10973942, PMID:10888878, PMID:11431533, PMID:12150907, PMID:12628165, PMID:16135753, PMID:21376232, PMID:23754282, PMID:23620051, PMID:24660806, PMID:24751536, PMID:29311685). Mediates monoubiquitination as well as ‘Lys-6’, ‘Lys-11’, ‘Lys-48’-linked and ‘Lys-63’-linked polyubiquitination of substrates depending on the context (PMID:19229105, PMID:20889974, PMID:25621951). Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating ‘Lys-63’-linked polyubiquitination of misfolded proteins such as PARK7: ‘Lys-63’-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation (PMID:17846173, PMID:19229105). Mediates ‘Lys-63’-linked polyubiquitination of a 22 kDa O-linked glycosylated isoform of SNCAIP, possibly playing a role in Lewy-body formation (PMID:11590439, PMID:11431533, PMID:19229105, PMID:11590439, PMID:15728840). Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy (PMID:20889974). Promotes the autophagic degradation of dysfunctional depolarized mitochondria (mitophagy) by promoting the ubiquitination of mitochondrial proteins such as TOMM20, RHOT1/MIRO1 and USP30 (PMID:19029340, PMID:19966284, PMID:23620051, PMID:24896179, PMID:25527291). Preferentially assembles ‘Lys-6’-, ‘Lys-11’- and ‘Lys-63’-linked polyubiquitin chains following mitochondrial damage, leading to mitophagy (PMID:25621951). Mediates ‘Lys-48’-linked polyubiquitination of ZNF746, followed by degradation of ZNF746 by the proteasome; possibly playing a role in the regulation of neuron death (PMID:21376232). Limits the production of reactive oxygen species (ROS). Regulates cyclin-E during neuronal apoptosis. In collaboration with CHPF isoform 2, may enhance cell viability and protect cells from oxidative stress (PMID:22082830). Independently of its ubiquitin ligase activity, protects from apoptosis by the transcriptional repression of p53/TP53 (PMID:19801972). May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity (PMID:11439185). May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. May represent a tumor suppressor gene. |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, WB, IHC, IF |
| Recommended Dilutions | WB: 1:500-1:5000, IHC: 1:500-1:1000, IF: 1:50-1:500 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human E3 ubiquitin-protein ligase parkin protein (1-465AA) |
| Specificity | PRKN antibody detects endogenous level of PRKN protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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