Anti-PRMT5 Antibody
419.18 $ – 243.68 $
Immunohistochemistry of paraffin-embedded human brain tissue with rabbit anti-PRMT5 antibody IGX-44166 at dilution 1:100.
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Catalog No
IGX-44166
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA (PMID:10531356, PMID:11152681, PMID:11747828, PMID:12411503, PMID:15737618, PMID:17709427, PMID:20159986, PMID:20810653, PMID:21258366, PMID:21917714, PMID:22269951, PMID:21081503). Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles (PMID:12411503, PMID:11747828, PMID:17709427). Methylates SUPT5H and may regulate its transcriptional elongation properties (PMID:12718890). Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. Methylates histone H2A and H4 ‘Arg-3’ during germ cell development. Methylates histone H3 ‘Arg-8’, which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage . Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR; this enhances EGFR ‘Tyr-1197’ phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation (PMID:21917714, PMID:21258366). Second, methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic activity (PMID:21917714). Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. Methylates HOXA9 (PMID:22269951). Methylates and regulates SRGAP2 which is involved in cell migration and differentiation (PMID:20810653). Acts as a transcriptional corepressor in CRY1-mediated repression of the core circadian component PER1 by regulating the H4R3 dimethylation at the PER1 promoter . Methylates GM130/GOLGA2, regulating Golgi ribbon formation (PMID:20421892). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PMID:25284789). Symmetrically methylates POLR2A, a modification that allows the recruitment to POLR2A of proteins including SMN1/SMN2 and SETX. This is required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination (PMID:26700805). Along with LYAR, binds the promoter of gamma-globin HBG1/HBG2 and represses its expression (PMID:25092918). Symmetrically methylates NCL (PMID:21081503). Methylates TP53; methylation might possibly affect TP53 target gene specificity (PMID:19011621). Involved in spliceosome maturation and mRNA splicing in prophase I spermatocytes through the catalysis of the symmetrical arginine dimethylation of SNRPB (small nuclear ribonucleoprotein-associated protein) and the interaction with tudor domain-containing protein TDRD6 . |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, IHC, IF |
| Recommended Dilutions | IHC: 1:20-1:200, IF: 1:50-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Protein arginine N-methyltransferase 5 protein (297-534AA) |
| Specificity | PRMT5 antibody detects endogenous level of PRMT5 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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