Anti-RAN Antibody
390.54 $ – 243.25 $
Western blot
All lanes: RAN antibody at 2µg/mL
Lane 1: HeLa whole cell lysate
Lane 2: Jurkat whole cell lysate
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-50028
Supplier
ImuGeX
Size
100 μL < 50 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs (PMID:10400640, PMID:8276887, PMID:8896452, PMID:8636225, PMID:8692944, PMID:9351834, PMID:9428644, PMID:9822603, PMID:26272610). Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:8896452, PMID:8636225, PMID:8692944, PMID:9351834, PMID:9428644, PMID:9822603, PMID:29040603, PMID:11336674, PMID:26272610). Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport (PMID:8896452, PMID:9351834, PMID:9428644). Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PMID:20485264). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603). Required for normal progress through mitosis (PMID:8421051, PMID:12194828, PMID:29040603). The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PMID:18591255). Acts as a negative regulator of the kinase activity of VRK1 and VRK2 (PMID:18617507). Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases (PMID:10400640). |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, WB, IHC |
| Recommended Dilutions | WB: 1:500-1:5000, IHC: 1:20-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human GTP-binding nuclear protein Ran protein (1-216AA) |
| Specificity | RAN antibody detects endogenous level of RAN protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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