Anti-Ripk1 Antibody
418.43 $ – 243.25 $
Immunoprecipitating Ripk1 in K562 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of anti-Ripk1 antibody IGX-45677 in K562 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: anti-Ripk1 antibody IGX-45677 (6µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (10µg)
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-45677
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways (PMID:25459879). Exhibits kinase activity-dependent functions that trigger cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival (PMID:31519887, PMID:31519886). Initiates ripoptocide which describes cell death that is dependent on RIPK1, be it apoptosis or necroptosis (PMID:31457011). Upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kB pathway (PMID:31519887, PMID:31519886). Specific conditions can however activate RIPK1, and its kinase activity then regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD-CASP8) and the complex IIb (RIPK1-RIPK3-MLKL) and these complexes respectively drive apoptosis or necroptosis, a regulated form of necrosis (PMID:29440439, PMID:30988283, PMID:31519887, PMID:31519886). Also involved in inflammatory response by promoting transcriptional production of pro-inflammatory cytokines, such as interleukin-6 (IL6) (PMID:31827281, PMID:31827280). During embryonic development suppresses apoptosis and necroptosis and prevents the interaction of TRADD with FADD thereby limiting aberrant activation of CASP8 (PMID:30867408, PMID:30185824, PMID:31511692). Phosphorylates DAB2IP at ‘Ser-728’ in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade . Required for ZBP1-induced NF-kappa-B activation and activation of NF-kappa-B by DNA damage and IR (PMID:12654725, PMID:19590578). |
| Host Species | Rabbit |
| Species Reactivity | Mouse, Human |
| Applications | ELISA, IP |
| Recommended Dilutions | IP: 1:200-1:2000 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Mouse Receptor-interacting serine/threonine-protein kinase 1 protein (1-656AA) |
| Specificity | Ripk1 antibody detects endogenous level of Ripk1 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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