Anti-sdeA Antibody
418.48 $ – 243.28 $
Western Blot
Positive WB detected in Recombinant protein
All lanes: sdeA antibody at 1:2000
Secondary:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-46297
Supplier
ImuGeX
Size
100 μL < 50 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Secreted effector that interferes with the host cell ubiquitin pathway and is required for intracellular bacterial replication. Catalyzes the ubiquitination of several mammalian Rab proteins (Rab33b, Rab1, Rab6a and Rab30) during L.pneumophila infection, without engaging the standard cellular enzyme cascade (E1 and E2) (PMID:29795346). Transfers an ADP-ribose moiety from NAD to the ‘Arg-42’ residue of ubiquitin in a reaction that releases nicotinamide (PMID:31330532, PMID:31330531, PMID:29795347). The modified ubiquitin is subsequently transferred to serine residues of the substrate protein via a phosphoribose linker that results in the release of AMP (PMID:27912065). Cannot ubiquitinate the endosomal Rab5 or the cytoskeletal small GTPase Rac1 (PMID:27049943). Also acts as a deubiquitinase (DUB), catalyzing the cleavage of three of the most abundant polyubiquitin chains (‘Lys-11’, ‘Lys-48’ and ‘Lys-63’) with a distinct preference for ‘Lys-63’ linkages; is thus able to efficiently remove ‘Lys-63’-linked polyubiquitin chains from the phagosomal surface. Is also able to remove NEDD8 from neddylated proteins, but is unable to recognize SUMO. The DUB activity of SdeA is important for regulating the dynamics of ubiquitin association with the bacterial phagosome, but is dispensable for its role in intracellular bacterial replication (PMID:26598703). |
| Host Species | Rabbit |
| Species Reactivity | Legionella pneumophila subsp |
| Applications | ELISA, WB |
| Recommended Dilutions | WB: 1:1000-1:5000 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Legionella pneumophila subsp Ubiquitinating/deubiquitinating enzyme SdeA protein (19-268AA) |
| Specificity | sdeA antibody detects endogenous level of sdeA protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
Related Products
-
418.48 $ – 243.28 $Buy now This product has multiple variants. The options may be chosen on the product page -
418.48 $ – 243.28 $Buy now This product has multiple variants. The options may be chosen on the product page -
418.48 $ – 243.28 $Buy now This product has multiple variants. The options may be chosen on the product page -
418.48 $ – 243.28 $Buy now This product has multiple variants. The options may be chosen on the product page