Anti-SIAH2 Antibody
417.46 $ – 242.68 $
Immunohinstochemical staining with rabbit anti-SIAH2 antibody IGX-45576 at 1:600 in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Catalog No
IGX-45576
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PMID:9334332, PMID:11483518, PMID:19224863). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PMID:9334332, PMID:11483518, PMID:19224863). Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes (PMID:9334332, PMID:11483518, PMID:19224863). Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (GPS2, POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP) (PMID:9334332, PMID:11483518, PMID:19224863). Mediates ubiquitination and proteasomal degradation of DYRK2 in response to hypoxia (PMID:22878263). It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes (PMID:9334332, PMID:11483518, PMID:19224863, PMID:22878263). Has some overlapping function with SIAH1 (PMID:9334332, PMID:11483518, PMID:19224863). Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 does not (PMID:12411493). Promotes monoubiquitination of SNCA (PMID:19224863). Regulates cellular clock function via ubiquitination of the circadian transcriptional repressors NR1D1 and NR1D2 leading to their proteasomal degradation (PMID:26392558). Plays an important role in mediating the rhythmic degradation/clearance of NR1D1 and NR1D2 contributing to their circadian profile of protein abundance (PMID:26392558). Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4 . |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, IHC, IF |
| Recommended Dilutions | IHC: 1:500-1:1000, IF: 1:200-1:500 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human E3 ubiquitin-protein ligase SIAH2 protein (1-72AA) |
| Specificity | SIAH2 antibody detects endogenous level of SIAH2 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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