Anti-SUFU Antibody
391.32 $ – 243.74 $
Western blot
All lanes: Suppressor of fused homolog antibody at 4µg/mL
Lane 1: HepG2 whole cell lysate
Lane 2: HeLa whole cell lysate
Lane 3: Mouse kidney tissue
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-48746
Supplier
ImuGeX
Size
100 μL < 50 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Negative regulator in the hedgehog/smoothened signaling pathway (PMID:10559945, PMID:10564661, PMID:10806483, PMID:12068298, PMID:12975309, PMID:27234298, PMID:15367681, PMID:22365972, PMID:24217340, PMID:24311597, PMID:28965847). Down-regulates GLI1-mediated transactivation of target genes (PMID:15367681, PMID:24217340, PMID:24311597). Down-regulates GLI2-mediated transactivation of target genes (PMID:24311597, PMID:24217340). Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome (PMID:10559945, PMID:10564661, PMID:10806483, PMID:24217340). Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein (PMID:10559945, PMID:10564661, PMID:10806483, PMID:24217340). Negative regulator of beta-catenin signaling . Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full-length form of GLI3 (GLI3FL) (PMID:24311597, PMID:28965847). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state (PMID:24311597, PMID:28965847). Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R (PMID:24311597, PMID:28965847). When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A) (PMID:24311597, PMID:28965847). Required for normal embryonic development . Required for the proper formation of hair follicles and the control of epidermal differentiation . |
| Host Species | Rabbit |
| Species Reactivity | Human, Mouse |
| Applications | ELISA, WB, IHC |
| Recommended Dilutions | WB: 1:500-1:2000, IHC: 1:20-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Suppressor of fused homolog protein (325-484AA) |
| Specificity | SUFU antibody detects endogenous level of SUFU protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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