Anti-TIMELESS Antibody
419.28 $ – 243.74 $
Immunohinstochemical staining with rabbit anti-TIMELESS antibody IGX-44975 at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Catalog No
IGX-44975
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication, DNA repair and in the regulation of the circadian clock (PMID:9856465, PMID:17141802, PMID:17296725, PMID:23418588, PMID:26344098). Required to stabilize replication forks during DNA replication by forming a complex with TIPIN: this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress (PMID:17141802, PMID:17296725). TIMELESS promotes TIPIN nuclear localization (PMID:17141802, PMID:17296725). Involved in cell survival after DNA damage or replication stress by promoting DNA repair (PMID:17141802, PMID:17296725, PMID:26344098, PMID:30356214). In response to double-strand breaks (DSBs), accumulates at DNA damage sites and promotes homologous recombination repair via its interaction with PARP1 (PMID:26344098, PMID:30356214). May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light (PMID:15798197). Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock (PMID:23418588). Negatively regulates CLOCK|NPAS2-ARTNL/BMAL1|ARTNL2/BMAL2-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus (PMID:9856465). May also play an important role in epithelial cell morphogenesis and formation of branching tubules . |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, IHC |
| Recommended Dilutions | IHC: 1:200-1:500 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Protein timeless homolog protein (1004-1131AA) |
| Specificity | TIMELESS antibody detects endogenous level of TIMELESS protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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