Anti-TRIP12 Antibody
391.46 $ – 243.82 $
Immunohistochemistry of paraffin-embedded human testis tissue with rabbit anti-TRIP12 antibody IGX-42962
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Catalog No
IGX-42962
Supplier
ImuGeX
Size
100 μL < 50 μL
Product Overview
Product Properties
Target Gene
Product Overview
| Description | E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair (PMID:19028681, PMID:22884692). Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardless of the presence of lysine residues in target proteins (PMID:19028681). Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of ‘Lys-63’-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes (PMID:22884692). In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress (PMID:20208519). In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation (PMID:20208519). Does not mediate ubiquitination of isoform p16-INK4a of CDKN2A (PMID:20208519). Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation (PMID:18627766). Ubiquitination and degradation of target proteins is regulated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins (PMID:20829358). Mediates ubiquitination of ASXL1: following binding to N6-methyladenosine methylated DNA, ASXL1 is ubiquitinated by TRIP12, leading to its degradation and subsequent inactivation of the PR-DUB complex (PMID:30982744). |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, IHC |
| Recommended Dilutions | IHC: 1:20-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human E3 ubiquitin-protein ligase TRIP12 protein (1-240AA) |
| Specificity | TRIP12 antibody detects endogenous level of TRIP12 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | Concentration can vary between different batches. |
| Storage Buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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