Anti-UBA5 Antibody
418.48 $ – 243.28 $
Western blot
All lanes: UBA5 antibody at 6µg/mL
Lane 1: Jurkat whole cell lysate
Lane 2: HeLa whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: Caco-2 whole cell lysate
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-48304
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | E1-like enzyme which specifically catalyzes the first step in ufmylation (PMID:15071506, PMID:18442052, PMID:25219498, PMID:20368332, PMID:27653677, PMID:26929408, PMID:27545674, PMID:30412706, PMID:27545681). Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP (PMID:20368332, PMID:27653677, PMID:26929408, PMID:30412706). Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer (PMID:27653677). Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding (PMID:29295865). Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism (PMID:27653677). Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress (PMID:32160526). Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes . |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, WB |
| Recommended Dilutions | WB: 1:500-1:2000 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human Ubiquitin-like modifier-activating enzyme 5 protein (291-404AA) |
| Specificity | UBA5 antibody detects endogenous level of UBA5 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
Related Products
-
418.48 $ – 243.28 $Buy now This product has multiple variants. The options may be chosen on the product page -
418.48 $ – 243.28 $Buy now This product has multiple variants. The options may be chosen on the product page -
418.48 $ – 243.28 $Buy now This product has multiple variants. The options may be chosen on the product page -
418.48 $ – 243.28 $Buy now This product has multiple variants. The options may be chosen on the product page