Anti-YTHDF2 Antibody
418.43 $ – 243.25 $
Western Blot
analysis of Jurkat whole cell lysate, HeLa whole cell lysate, HepG2 whole cell lysate
All lanes: YTHDF2 antibody at 2.7µg/mL
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
IGX-48131
Supplier
ImuGeX
Size
100 μg < 50 μg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability (PMID:24284625, PMID:26046440, PMID:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing (PMID:24284625, PMID:22575960, PMID:25412658, PMID:25412661). Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT and ribonuclease P/MRP complexes, depending on the context (PMID:24284625, PMID:26046440, PMID:27558897, PMID:30930054). M6A-containing mRNAs containing a binding site for RIDA/HRSP12 (5′-GGUUC-3′) are preferentially degraded by endoribonucleolytic cleavage: cooperative binding of RIDA/HRSP12 and YTHDF2 to transcripts leads to recruitment of the ribonuclease P/MRP complex (PMID:30930054). Other m6A-containing mRNAs undergo deadenylation via direct interaction between YTHDF2 and CNOT1, leading to recruitment of the CCR4-NOT and subsequent deadenylation of m6A-containing mRNAs (PMID:27558897). Required maternally to regulate oocyte maturation: probably acts by binding to m6A-containing mRNAs, thereby regulating maternal transcript dosage during oocyte maturation, which is essential for the competence of oocytes to sustain early zygotic development . Also involved in hematopoietic stem cells specification by binding to m6A-containing mRNAs, leading to promote their degradation (PMID:30065315). Also acts as a regulator of neural development by promoting m6A-dependent degradation of neural development-related mRNA targets . Regulates circadian regulation of hepatic lipid metabolism: acts by promoting m6A-dependent degradation of PPARA transcripts (PMID:30428350). Regulates the innate immune response to infection by inhibiting the type I interferon response: acts by binding to m6A-containing IFNB transcripts and promoting their degradation (PMID:30559377). Also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5′-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation (PMID:26458103). (Microbial infection) Promotes viral gene expression and replication of polyomavirus SV40: acts by binding to N6-methyladenosine (m6A)-containing viral RNAs (PMID:29447282). (Microbial infection) Promotes viral gene expression and virion production of kaposis sarcoma-associated herpesvirus (KSHV) at some stage of the KSHV life cycle (in iSLK.219 and iSLK.BAC16 cells) (PMID:29659627). Acts by binding to N6-methyladenosine (m6A)-containing viral RNAs (PMID:29659627). |
| Host Species | Rabbit |
| Species Reactivity | Human |
| Applications | ELISA, WB, IHC, IF |
| Recommended Dilutions | WB: 1:2000-1:5000, IHC: 1:20-1:200, IF: 1:50-1:200 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Human YTH domain-containing family protein 2 protein (201-500AA) |
| Specificity | YTHDF2 antibody detects endogenous level of YTHDF2 protein |
Product Properties
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone ID | |
| Concentration | 1 mg/mL |
| Storage Buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. |
| Formulation | Liquid |
| Purification | This antibody is immunogen affinity purified. |
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. |
| Reactivity Life | 12 months when stored properly according to instructions (provided antibody remains sterile). |
Target Gene
NA
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