TaaI (Tsp4CI)
433.01 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-T0290
Supplier
US Biologicals
Size
200U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | 5′-A C N^G T-3′ 3′-T G^N C A-5′ Unit Definition: One unit is defined as the amount of Taal required to digest 1ug of lambda DNA in 1 hour at 65°C in 50ul of reaction buffer. Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol Storage Buffer: 10mM Tris-HCl, pH 7.5, 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol Methylation Effects: Dam: never overlaps – no effect Dcm: never overlaps – no effect CpG: may overlap – cleavage impaired EcoKl: may overlap – effect not determined EcoBl: may overlap – effect not determined Number of Recognition Sites in DNA: Lambda: 187, PhiX174: 15 pBR322: 14, pUC57: 8, pUC18/19: 8, pTZ19R/U: 7, M13mp18/19: 31 Stability during Prolonged Incubation: A minimum of 0.2 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 65°C. Compatible Ends: Hpy188l Incubation Temperature: 65ºC Thermal Inactivation: Enzyme is inactivated by incubation at 80°C for 20 minutes. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with TaaI. Ligation/Recleavage Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a facrtor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded or double-stranded labeled oligonucleotide was observed after incubation with 10units of Taal for 4 hours. Storage and Stability: Store at -20°C. Aliquots are stable for 6 months after receipt. |
| Source Antigen | Thermus aquaticus Vn 4–311 |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
