β-Galactosidase (E. coli), Recombinant, ELISA Control
566.90 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-G1041-05H
Supplier
US Biologicals
Size
100 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Recombinant DNA technology allows the addition of short pieces of well-defined tags, “peptides” or proteins at the amino or c-terminus of target genes, which can provide ‘affinity handles’ designed to bind specific matrices. Therefore, tags enable a selective identification and purification of the protein of interest. Eukaryotic genes are often cloned into E. coli B-galactosidase (lacZ) gene, resulting in the expression of a desired protein as a fusion hybrid with B-galactosidase (1-3). Anti-B-galactosidase antibodies allow a simple isolation of fusion proteins directly from the crude bacterial lysates, using immunoaffinity chromatography (1-5) or used in immunoprecipitation. Anti-Beta galactosidase can also be used for the immunocytochemical detection of B-galactosidase in cells and tissues that express transfected bacterial lacZ gene (6). Ant-beta galactosidase may be used in various immunoassays to identify the expression of beta-galactosidase fusion protein. Source: Recombinant purified E. coli Beta-galactosidase protein Applications: Suitable for use in ELISA as a control. This preparation is not biologically active. This preparation is intended for qualitative purpose and not to serve as standard of known concentration. Other applications not tested. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Purified |
| Purity | Purified |
| Form | Supplied as a liquid in PBS, pH 7.2. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
