Actin, beta Human
656.57 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-A0760-44F
Supplier
US Biologicals
Size
100 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (gsmooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western Blots. Biological Activity: Not tested. If the protein is stored and used properly can be used for biological assays. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1ug/ml Used to coat plates Western Blot: 100-500ng/lane in SDS-PAGE sample buffer (reduced) and load as needed. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Human platelets cells. |
| Reactivity | |
| Cross Reactivity | Human |
| Applications | |
| Molecular Weight | 43 |
Product Properties
| Storage | -20°C |
| Grade | Purified |
| Purity | Purified (~99%). |
| Form | Supplied as a lyophilized powder from 5mM Tris. pH 8, 0.2mM calcium chloride, 0.2mM ATP, 5% sucrose, 1% dextran. Reconstitute with 100ul sterile ddH2O |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
