Actin, beta Human (non-muscle) Western Blot Positive Control
594.35 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-A0760-47B
Supplier
US Biologicals
Size
100ul
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots. Source of Antigen and Antibodies Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: load 10ul/lane. Note: SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the A0760-47B solution prior to heating and loading on gels. This preparation is not biologically active. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | |
| Reactivity | |
| Cross Reactivity | Human |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Purified |
| Purity | Purified by affinity chromatography to >95% purity. |
| Form | Supplied as a lyophilized powder from PBS, 0.05% sodium azide. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
