AdeI (DraIII)
418.42 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-A0859-75
Supplier
US Biologicals
Size
500U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | 5′-C A C N N N^G T G-3′ 3′-G T G^N N N C A C-5′ Source: Alcaligenes denitrificans SS 3–028 Concentration: 10u/ul Unit Definition: One unit is defined as the amount of AdeI required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Incubation Temperature: 37°C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: Supplied as a liquid in 10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Supplied with: R1625-02: Restriction Enzyme Buffer C, 10X: Dilute to 1X. 1X buffer compostion is 10mM Tris-HCl (pH 7.5), 10mM MgCl2, 50mM sodium chloride and 0.1mg/ml BSA. Incubate at 37°C R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37°C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Enzyme Properties: Star Activity: An excess of AdeI (20u/ug DNA x 1 hour) may result in star activity. Methylation Effects: Dam, Dcm: Never overlaps – No effect. CpG: May overlap – cleavage impaired. EcoKl, EcoBl: May overlap – Effect not determined. Stability during Prolonged Incubation: A minimum of 0.5units of AdeI is required for complete digestion of 1ug of DNA in 16 hours at 37°C. Digestion of Agarose-embedded DNA: A minimum of 10units of AdeI is required for complete digestion of 1ug of agarose- embedded lambda DNA in 16 hours. Thermal Inactivation: AdeI is not inactivated by incubation at 80°C for 20min (see Protocol for Inactivation Procedure. Number of Recognition Sites in DNA: Lambda: 10 PhiX174, pTZ19R/U, M13mp18/19: 1 pBR322, pUC18/19, pUC57: 0 |
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| Reactivity | |
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Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
