Aldo-keto Reductase Family 1 Member C1, Recombinant, Human (AKR1C1, 20-alpha-hydroxysteroid Dehydrogenase, 20-alpha-HSD, 2-alpha-HSD, C9, Chlordecone Reductase Homolog HAKRC, Indanol Dehydrogenase, Dihydrodiol Dehydrogenase 1/2, DD1, DD1/DD2, DDH, DDH1, H-37, HAKRC, High-affinity Hepatic Bile Acid-binding Protein, HBAB, MBAB, MGC8954, Trans-1,2-dihydrobenzene-1,2-diol Dehydrogenase)
539.99 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-A1334-70L
Supplier
US Biologicals
Size
100 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | AKR1C1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant protein corresponding to aa1-323 of human AKR1C1 6x His tagged expressed in E. coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY Biological Activity: Specific activity is ~0.15-0.2units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH/minute at 25°C. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 38.9 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ~90% (SDS-PAGE) |
| Form | Supplied as a liquid in 20mM Tris HCl pH 8.0, 1mM DTT, 20% glycerol |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
