Alkaline Phosphatase, Bovine (AP)
420.69 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-A1355-65B
Supplier
US Biologicals
Size
1 mg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Alkaline Phosphatase is Dimeric protein having a molecular weight of 140kD, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity. Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity. Activity: ~1000U/mg (pH 9.6, 37°C, with 0.025M glycine, 10% glycerol as buffer). Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Calf Intestine |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 140 |
Product Properties
| Storage | 4°C Do not freeze |
| Grade | Highly Purified |
| Purity | ≥ 95% pure by Gel Filtration. |
| Form | Supplied as a liquid in 5mM MgCl2, 0.1mM ZnCl2, 5mM TRIS, pH 7.0, 50% glycerol. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
