Angiostatin Kringles 1-3, Recombinant, Human
423.52 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-A2294-33
Supplier
US Biologicals
Size
20 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Recombinant human Angiostatin is a single, non-glycosylated polypeptide chain containing 259 amino acids and having a molecular mass of ~30kD. rHuAng K1-3 is a proteolytic fragment of plasminogen containing the first three kringle structures. It is more active relative to kringles 1-4. rHuAng K1-3 is a specific inhibitor of endothelial cell growth and angiogenesis. It reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. Amino Acid Sequence: VYLSECKTGN GKNYRGTMSK TKNGITCQKW SSTSPHRPRF SPATHPSEGL EENYCRNPDN DPQGPWCYTT DPEKRYDYCD ILECEEECMH CSGENYDGKI SKTMSGLECQ AWDSQSPHAH GYIPSKFPNK NLKKNYCRNP DRELRPWCFT TDPNKRWELC DIPRCTTPPP SSGPTYQCLK GTGENYRGNV AVTVSGHTCQ HWSAQTPHTH NRTPENFPCK NLDENYCRNP DGKRAPWCHT TNSQVRWEYC KIPSCDSSP Biological Activity: Activity is assayed on anti-proliferation and anti-migration of endothelial cells in vitro and anti-angiogenesis in vivo. Specific Activity: 0.55x10e5U/mg based on anti-migration of endothelial cells in vitro. Protein Content: Carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 2.08 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (Intelligenetics). 2. Analysis by RP-HPLC, using a standard solution of Ang K1-3 as a reference. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O, 0.1% BSA. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 30 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | >95% as determined by RP-HPLC and SDS-PAGE; Purified by Proprietary chromatographic techniques. |
| Form | Supplied as a lyophilized powder from 20mM sodium acetate, pH 5.5, 4% mannitol. Reconstitute with sterile ddH20 or aqueous buffer, 0.1% BSA to 0.1-1mg/ml. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
